9th International Conference on Food Pathogen and Food Safety
Paris, France
Atefeh Nasri
Department of Veterinary Biomedical Science, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada
Title: In vitro estrogenic, cytotoxic, and genotoxic profiles of the phytoestrogen 8-prenylnaringenin (8-PN) and the xenoestrogen tartrazine
Biography
Biography: Atefeh Nasri
Abstract
Unlike the toxicological risks to human health emphasized for synthetic environmental estrogens, in the case of phytoestrogens, the health-promoting effects have been in the limelight. To find out whether there is indeed a clear distinction between these two compound groups, the estrogenic, cytotoxic, and genotoxic profiles of two compounds with estrogen-like characteristics, the phytoestrogen 8-PN and the synthetic xenoestrogen tartrazine (a food additive), were analyzed in this study. As assessed by a yeast bioreporter assay and estrogen-dependent proliferative response in a human mammary gland adenocarcinoma cell line (MCF-7) and a human meningioma cell line (CH-157MN), 8-PN showed higher estrogen-like activity than tartrazine. After 24-h incubation on MCF-7 and CH157-MN cells, both compounds exhibited low cytotoxicity in the lactate dehydrogenase assay and low genotoxicity in the micronucleus assay. These results demonstrate that 8-PN and tartrazine possess variable estrogenic activity but display little cellular toxicity in short-term tests in vitro. No fundamental difference between phytoestrogens and synthetic xenoestrogens could thus be established.
Keywords: Phytoestrogens, Xenoestrogens, Estrogenic activity, Cytotoxicity, Genotoxicity, 8-prenylnaringenine, Tartrazine